Glucose Regulation of Gene Transcription

Glucose regulation of gene transcription.

Nutrient gene regulation is an important adaptation allowing survival on intermittent food supplies. This adaptative process exists in all species from yeast to mammals. Glucose, the most abundant monosaccharide in nature, provides a very good example of how organisms have developed regulatory mechanisms to cope with a fluctuating level of nutrient supply. In yeast, glucose facilitates its own ...

Short-term regulation of insulin gene transcription by glucose.

Whereas short-term regulation of insulin biosynthesis at the level of translation is well accepted, glucose-dependent transcriptional control is still believed to be a long-term effect occurring after more than 2 hr of glucose stimulation. Because pancreatic beta cells are exposed to elevated glucose levels for minutes rather than hours after food uptake, we hypothesized the existence of a shor...

Feedback regulation of glucose transporter gene transcription in Kluyveromyces lactis by glucose uptake.

In the respirofermentative yeast Kluyveromyces lactis, only a single genetic locus encodes glucose transporters that can support fermentative growth. This locus is polymorphic in wild-type isolates carrying either KHT1 and KHT2, two tandemly arranged HXT-like genes, or RAG1, a low-affinity transporter gene that arose by recombination between KHT1 and KHT2. Here we show that KHT1 is a glucose-in...

Regulation of ribosomal gene transcription.

In eukaryotic cells, ribosomal RNA (rRNA) gene is transcribed by RNA polymerase I (pol I) in the nucleolus as a large (40 S-47 S) precursor RNA (pre-rRNA) (for reviews, see refs. [1,2]). The mature rRNA species (28 S, 18 S and 5.8 S) are formed after a series of specific endonucleolytic cleavage reactions (for a review, see ref. [3]). The rRNA gene (rDNA) is highly reiterated and is arranged in...

Down Regulation of DHFR Promoter Activity by PML Transcription Repressor